Stemolecule™ Thiazovivin

Catalog Size Price Quantity
04-0017 1 mg $209.00
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04 0017 Figure 1

Product Overview

Thiazovivin is a selective, cell permeable small molecule that directly targets Rho-associated kinase (ROCK)1. In addition, Thiazovivin protects human embryonic stem cells (hESCs) in the absence of ECM by regulating E-cadherin mediated cell-cell interaction1. This observation suggests that Thiazovivin promotes cell survival. In other studies Thiazovivin, in combination with inhibitors of the TGF-β receptor and MEK pathway, has shown to improve reprogramming efficiency by more than 200-fold2.


Product Specifications

Size

1 mg

Chemical Name

N-benzyl-2-(pyrimidin-4-ylamino)thiazole-4-carboxamide

Chemical Formula

C15H13N5OS

Molecular Weight

311.36

CAS Number

1226056-71-8

Purity

Greater than 98% by HPLC analysis 

Formulation

Light brown powder

Solubility

For a 10 mM concentrated stock solution of Thiazovivin, reconstitute the compound by adding 321.2 μl of DMSO to the entire contents of the vial. If precipitate is observed, warm the solution to 37°C for 2 to 5 minutes. For use in cell culture, warm the medium just prior to adding the reconstituted compound. Once the compound is added, mix and filter-sterilize the medium using a 0.2 µM low-protein binding filter. Thiazovivin is soluble in DMSO at 100 mM.

Storage and Stability

Store powder at 4°C protected from light. Following reconstitution, store aliquots at -20°C. Stock solutions are stable for 6 months when stored as directed. 

Quality Control

The purity of Thiazovivin was determined by HPLC analysis. The accurate mass was determined by mass spectrometry. No acute cytotoxicity was observed in mouse embryonic stem cells following a 6 hour exposure to 1 nM - 100 µM of Thiazovivin.


Specification Sheets

Safety Data Sheets

References

  1. Xu, Y., Zhu, X., Hahm, H.S., Wei, W., Hao, E., Hayek, A., and Ding, S. (2010) Revealing a core signaling regulatory mechanism for pluripotent stem cell survival and self-renewal by small molecules. Proc Natl Acad Sci USA 107: 8129-8134.
  2. Lin, T., Ambasudhan, R., Yuan, X., Li, W., Hilcove, S., Abujarour, R., Lin, X., Hahm, H.S., Hao, E., Hayek, A., and Ding, S. (2009) A chemical platform for improved induction of human iPSCs. Nat Methods 6: 805-808.

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