Stemolecule™ Cyclopamine

Catalog Size Price Quantity
04-0022 2 mg $119.00
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04-0022 Figure 1

Product Overview

Stemolecule Cyclopamine is a steroid alkaloid isolated from the corn lily (Veratrum californicum) and originally identified as a teratogenic agent1. Cyclopamine has since been identified as a specific inhibitor of hedgehog singaling by direct binding to the heptahelical bundle of Smoothened2. Hedgehog signaling is involved in embryogenesis as well as cancer progression3. Cycoplamine has been utilized as a small molecule inducer of stem cell differentiation towards definitive endoderm pancreatic islet cells, as a modulator of cell proliferation, and as an anticancer drug4-6


Product Specifications

Size

2 mg

Alternate Names

(3S,3'R,3'aS,6'S,6aS,6bS,7'aR,9R,11aS,11bR)-3',6',10,11b tetramethylspiro[2,3,4,6,6a,6b,7,8,11,11a-decahydro-1H-benzo[a]fluorene-9,2'-3a,4,5,6,7,7a-hexahydro-3H-furo[3,2-b]pyridine]-3-ol

Chemical Formula

C27H41NO2

Molecular Weight

411.62

CAS Number

4449-51-8

Purity

Greater than 99% by TLC analysis 

Formulation

White crystalline solid or clear solid film at bottom of vial

Solubility

For a 10 mM concentrated stock solution of Cyclopamine, reconstitute the compound by adding 485.9 μl of DMSO to the entire contents of the vial. If precipitate is observed, warm the solution to 37°C for 2 to 5 minutes. For cell culture, the media should be prewarmed prior to adding the reconstituted compound. Note: for most cells, the maximum tolerance to DMSO is less than 0.5%. This molecule is soluble in DMSO at 100 mM, ethanol at 49 mM, and methanol at 17 mM.

 Storage and Stability

Store powder at 4°C protected from light. Following reconstitution, store aliquots at -20°C. Stock solutions are stable for 6 months when stored as directed. 

Quality Control

The purity of Cyclopamine was determined by TLC analysis. The accurate mass was determined by mass spectrometry. Cellular toxicity of Cyclopamine was tested on mouse embryonic stem cells. 


Specification Sheets

Safety Data Sheets



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