Stemolecule™ IDE-2

Catalog Size Price Quantity
04-0027 2 mg $199.00
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Product Overview

Stemolecule IDE-2 was identified in a high throughput screen as an inducer of definitive endoderm (IDE) from embryonic stem (ES) cells. IDE-2 is able to generate Sox17+/FoxA2+ cells to an equal or greater efficiency than that of protein (i.e. Activin A or Nodal) IDEs in mouse or human ES cell cultures. It  functions in part via activation of TGF-β signaling, as evident by Smad2 phosphorylation. IDE-2 chemically induced endoderm can continue to differentiate into pancreatic progenitors in vitro and contribute to gut tube formation in vivo when injected into the developing gut tube of mouse embryos1.


Product Specifications

Size

2 mg

Alternate Name

7-(2-cyclopentylidenehydrazinyl)-7-oxoheptanoic acid

Chemical Formula

C12H20N2O3

Molecular Weight

240.30

CAS Number

1136466-93-7

Purity

Greater than 99% by HPLC analysis 

Formulation

White Powder

Solubility

For a 10 mM concentrated stock solution of IDE-2, reconstitute the compound by adding 832.3 μl of DMSO to the entire contents of the vial. If precipitate is observed, warm the solution to 37°C for 2 to 5 minutes. For cell culture, the media should be prewarmed prior to adding the reconstituted compound. Note: for most cells, the maximum tolerance to DMSO is less than 0.5%. This molecule is soluble in DMSO at 100 mM. 

Storage and Stability

Store powder at 4°C protected from light. Following reconstitution, store aliquots at -20°C. Stock solutions are stable for 6 months when stored as directed.

Quality Control

The purity of IDE-2 was determined by HPLC analysis. The accurate mass was determined by mass spectrometry. Cellular toxicity of IDE-2 was tested on mouse embryonic stem cells. 


Specification Sheets

Safety Data Sheets



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